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毛细管电泳高频电导法测定几种中药中的丁香酚

Determination of Eugenol in Several Chinese Herbal Medicines by Capillary Electrophoresis with High Frequency Conductivity Method

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【作者】 林伟丰吴小林陈缵光李全文蔡沛祥莫金垣

【Author】 LIN Wei-Feng~(1),WU Xiao-Lin~(2),CHEN Zuan-Guang~(3*), LI Quan-Wen~(3),CAI Pei-Xiang~(1),MO Jin-Yuan~(1)(1.School of Chemistry,Sun Yat-Sen University,Guangzhou 510275,China; 2.The Third Affiliated Hospital,Sun Yat-Sen University,Guangzhou 510630,China;3.School of Pharmaceutical Sciences,Sun Yat-Sen University,Guangzhou 510089,China)

【机构】 中山大学化学院中山大学附属笫三医院中山大学药学院中山大学化学院 广州510275广州510630广州510089广州510275

【Abstract】 A method based on capillary electrophoresis with a high frequency conductivity method was(developed) for the determination of eugenol in several Chinese herbal medicines from different producing areas.In this study,some key factors for separation and detection were studied and the best analysis conditions were obtained.In the buffer system of 2.0 mmol/L triethylamine +0.74 mol/L methanol at separation potential of 18.0 kV,the optimal separation and detection was obtained.Under the chosen conditions,eugenol was eluted within 7 min.The linear range of eugenol was 2.0—540 μg/mL,the limit of detection was 0.6 μg/mL,and the recovery ranged from 93.3% to 106%.

【基金】 国家自然科学基金(批准号:20375049);广东省自然科学基金重点项目(批准号:021808)资助
  • 【文献出处】 高等学校化学学报 ,Chemical Journal of Chinese Universities , 编辑部邮箱 ,2006年11期
  • 【分类号】R284
  • 【被引频次】18
  • 【下载频次】224
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