【摘要】 利用两亲性线性-超支化多臂共聚物聚乙二醇-聚乙烯亚胺-聚谷氨酸苄酯(PEG-PEI-PBLG)在水溶液中自组装为阳离子囊泡.利用透射电镜、动态光散射、静态光散射和zeta电位仪对囊泡结构进行了表征.PEG-PEI-PBLG囊泡具有双分子层结构,壁厚5~10nm,直径在100nm左右.由于PEI在水溶液中的质子化作用,囊泡表面携带有正电荷,其表面电势为+25.2mV,因此PEG-PEI-PBLG阳离子囊泡具有担载负电性蛋白的能力.更多还原

【Abstract】 Amphiphilic linear-hyperbranched multi-arm copolymer poly(ethylene glycol)-polyethylenimine-poly(γ-benzyl L-glutamate)(PEG-PEI-PBLG) can self-assemble into cationic vesicles in aqueous solution. TEM, DLS, SLS and zeta potential measurement were used to characterize the structure of the PEG-PEI-PBLG vesicles. The vesicles were constituted of 5—10 nm bimolecular layers with average diameter in 100 nm and surface zeta potential of +25.2 mV. The positive charges on vesicles surface were caused by the protonation of PEI in aqueous solution. The cationic vesicles have an ability to carry negatively charged proteins.更多还原