【摘要】 将电喷雾解吸电离质谱(DESI-MS)用于临床尿样的分析,优化了电喷雾溶剂流速、电喷雾电压和喷雾锥距离等重要参数.采用普通滤纸作为样品载体,在不需要样品预处理的前提下同时快速测定了临床尿样中的钾、钠、尿素、尿酸、丙酮酸和肌苷等多种成分,并对各种成分的主要离子进行了串联质谱鉴定.DESI-MS在进行多组分同时测定时不需要进行样品预处理,缩短了测定时间,单个样品的分析时间不到1 m in.同时,采用内标法对所测定组分进行了半定量分析.更多还原

【Abstract】 DESI-MS was applied to direct simultaneous detection of multiple components of clinic urine samples without any sample pretreatment.Under the optimized working conditions,such as ESI infusion rate,ESI high voltage, incident angle,collective angle,tube lens voltage,etc.,for both DESI and LTQ mass spectro-(meter),the main components of urine including urea,pyruvic acid,creatinine,oxalic acid,alloxane,menadione were detected as the protonated molecules in positive detection mode using methanol-water(volume ratio(1∶1)) as the spray solvent in the low mass range(15—200 amu).Sodium and potassium were also detected but mainly in the form of clusters with urea and creatinine.In each case,the corresponding ions were isolated in the ion trap mass analyzer and underwent a collision-induced dissociation process,which yielded characteristic fragments in tandem mass spectra and resulted in confident validation of the analytical results.The average time for single sample analysis was less than 1 min since it required no sample pretreatment.Furthermore,the semi-quantitative analysis of the components was also demonstrated by adding serine as an internal standard(into) the samples.更多还原