【摘要】 为了开发新型多功能天然高分子荧光材料,合成出一种新型含有环氧丙氧基荧光素(EPF)基团的水溶性壳聚糖衍生物GCS-EPF,并用IR,1H NMR,UV光谱和荧光光谱等手段进行结构和发光性能的研究.结果表明,修饰后水溶性壳聚糖(GCS)的水溶液、固体粉末和薄膜在520 nm附近具有较强的荧光发射,其荧光强度不仅在0~60℃时对温度有较快敏感响应,同时在pH=0~13.5时对pH也有较快敏感响应,具有双重敏感响应,因此可将其作为温度荧光探针和pH荧光探针的高分子材料.更多还原

【Abstract】 A novel method for attaching 3-epoxypropoxy fluorescein(EPF) to water soluble chitosan(GCS) and its dual sensitive quality of fluorescence were investigated.GCS bearing EPF was characterized by the methods of IR,()~1H NMR,UV and fluorescence spectra,respectively.The chemiluminescent and photophysical behavior of GCS-EPF were studied,and the pH and temperature dependences of fluorescence of GCS-EPF were investigated in detail.From the results,we found that the emission wavelengths of GCS-EPF in solid and film states were larger than that in solution state,which may be due to the formation of an excimer between chromophore moieties on a chitosan chain.At 0—60 ℃,the maximum fluorescence intensity of GCS-EPF(decreased) on heating and the relationship between fluorescence intensity and the change of temperature was(linear);At pH=0—13.5,its fluorescence intensity increased with basification and completely disappeared at very acidic pH.更多还原