节点文献

基于层-层自反应的葡萄糖氧化酶有序多层膜电极

Ordered Multilayer Film Electrode Containing Glucose Oxidase Based on Layer-by-layer Self-reaction

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 孙莹莹赵爽杨微微孙长青

【Author】 SUN Ying-Ying,ZHAO Shuang,YANG Wei-Wei,SUN Chang-Qing~*(College of Chemistry,Jilin University,Changchun 130012,China)

【机构】 吉林大学化学学院吉林大学化学学院 长春130012长春130012

【摘要】 以胱胺修饰的金电极为基础电极,利用席夫碱反应使经高碘酸根氧化的葡萄糖氧化酶在该电极表面进行自身的层-层有序组装.用电化学交流阻抗法对多层酶膜形成过程的跟踪结果表明,该多层酶膜的生长是一个逐步形成的均匀过程.用循环伏安法和I-t曲线法研究了该酶电极对葡萄糖的电催化氧化.实验结果表明,当采用羟基二茂铁作为人工电子转移媒介体时,该酶电极对葡萄糖具有很好的电催化氧化功能.该传感器制作简便,响应迅速,性能稳定,催化电流与葡萄糖浓度在一定范围内成正比,并且可以通过控制葡萄糖氧化酶的组装层数来调节该生物传感器的灵敏度与检测限.

【Abstract】 Highly stable glucose oxidase(GOx) multilayer films were fabricated on cystamine modified gold electrode surfaces by layer-by-layer self-reaction of periodate-oxidized glucose oxidase.The fabricated process was followed by electrochemical impedance spectroscopy.The experimental results showed that the film was homogeneous and formed in an ordered manner.The gold electrode modified with the GOx multilayers showed excellent electrocatalytical response to the oxidation of glucose when ferrocenemethanol was used as an artificial redox mediator.From the analysis of voltammetric signal,the coverage of active enzyme on the electrode surface was estimated,which had a linear relationship with the number of GOx besides the first layer.This suggested that the analytical performance such as sensitivity,detection limit,and so on,was tunable by controlling the number of the fabricated GOx layer.In addition,the biosensor exhibited a good reproducibility and stability.

【基金】 国家“九七三”计划项目(批准号:G2000078102);国家自然科学基金(批准号:20175007);教育部博士学科点基金(批准号:20020183007);吉林大学超分子结构与材料教育部重点实验室基金资助
  • 【文献出处】 高等学校化学学报 ,Chemical Journal of Chinese Universities , 编辑部邮箱 ,2006年05期
  • 【分类号】TP212.3
  • 【被引频次】27
  • 【下载频次】232
节点文献中: 

本文链接的文献网络图示:

本文的引文网络