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人卵巢癌耐药细胞株的比较蛋白质组分析

Comparative Analysis of Proteomes for Chemoresistance in Human Ovarian Adenocarcinoma Cell Line,COC₁/DDP

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【Author】 LLei~(1,2,3),LIU Zhi-Qiang~(1*),LI Li~(1),LIU Ning~(1),LIU Shu-Ying~(1)(1.Laboratory of New Drug Research and Mass Spectrometry,Changchun Institute of Applied Chemistry,Changchun Center of Mass Spectrometry,Changchun 130022,China;2.Department of Chemistry,Yan′an University,Yan′an 716000,China;3.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China)

【机构】中国科学院长春应用化学研究所新药研究实验室长春质谱中心；中国科学院长春应用化学研究所新药研究实验室长春质谱中心长春130022；延安大学化学系；延安716000；中国科学院研究生院；北京100039；长春130022；

【Abstract】 The proteomic maps of human ovarian adenocarcinoma cell line in Human COC₁ and its cell subline COC₁/DDP were produced by 2-DE SDS-PAGE.The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment.Approximate 1 300 protein spots were resolved in the two ranges of pI（3—10）.The spots which have 10-fold difference in light density were digested by in-gel（enzyme）,identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and finally detected by Profound and Mascot search engine in IDI databases.Seven proteins with obviously altered abundances were detected specifically,including four up-regulated protein spots and three down-regulated protein spots.The proteins differentially expressed in the parent cell line COC₁ and its subline COC₁/DDP were considered to be important for the therapy and pathogenesis.更多还原