【摘要】 利用HPLC-DAD提供的二维化学数据信息,通过多组分光谱相关色谱方法及化学计量学分辨技术,对相同试验条件下所得的当归补血汤及其单味药的色谱组分进行了比较和归属分析.结果表明,在当归补血汤色谱响应值较高的21个色谱峰中,8个色谱峰的组分来自当归,6个色谱峰的组分来自黄芪,7个色谱峰的组分同时来源于当归和黄芪.其中一些色谱峰簇中的其它组分发生了消长变化,既有新物质的产生,又有原有化学成分的消失,说明中药复方配伍的药效物质基础不仅是各单味药化学成分的总和,还有单味药在复方配伍过程中各种成分的相互作用.本方法为中药复方与单味药化学组分的比较及归属分析,尤其对重叠色谱峰簇的组分比较分析提供了方便、快捷及可行的研究方法.更多还原

【Abstract】 In this study,a compound medicine of Dangguibuxuetang and its two involved single medicines of Danggui(Radix Angelica sinensis) and Huangqi(Radix Astragali) were compared for their relevant constituents after the combination of hyphenated chromatography and multicomponent spectral correlative chromatography,based on the two-dimensional spectrochromatographic fingerprints of the three medicinal objects obtained from HPLC-DAD.It was found that of twenty-one high peaks in Dangguibuxuetang,eight originate from Radix Angelica sinensis,six from Radix Astragali,and seven from both Radix Angelica sinensis and Radix Astragali.A little of other components in the investigated overlapping peaks varied.Moreover,new chemical ingredients appeared just in spectrochromatographic fingerprints of Dangguibuxuetang,and some existing in those of single medicines contrarily disappeared after the compatibility,as opposite in Radix Angelica sinensis and Radix Astragali.It suggested that there are certain interactions of the chemical ingredients in compound medicine besides their total sum effect of single medicines.This proposed approach was quite convenient and reliable to make a quick comparison and analysis of the relevant constituents in spectrochromatographyic fingerprints of the compound and single medicines,especially for the comparison of two overlapping peaks.更多还原