【摘要】 目的探讨碱性成纤维细胞生长因子(bFGF)对大鼠胰腺干细胞增殖的影响。方法大鼠胰腺干细胞经体外纯化、扩增,观察其生长特性,免疫组化法检测CK-19和PDX-1的表达。研究不同浓度的bFGF对大鼠胰腺干细胞生长曲线的影响。结果原代培养的细胞经差速贴壁后,得到纯化的胰腺干细胞。免疫组化显示大鼠胰腺干细胞呈CK-19阳性和PDX-1阴性。对照组(无bFGF)、bFGF浓度分别为5,10,20 ng/ml各组的细胞数分别是3.29×105/ml,3.47×105/ml,3.83×105/ml和3.83×105/ml。加bFGF 3组的细胞数与对照组比较均有显著性差异(均P<0.01),10,20 ng/ml bFGF组细胞数与5 ng/ml bFGF组细胞数比较差异有显著性(P<0.01);10 ng/ml和20 ng/ml bFGF组差异无显著性(P>0.05)。结论5,10,20 ng/ml浓度的bFGF可明显促进SD大鼠胰腺干细胞增殖。更多还原

【Abstract】 Objective To investigate the effect of basic fibroblast growth factor(bFGF) on proliferation of pancreatic stem cell(PSC) of SD rat.Methods PSCs were cultured in vitro after being purified.CK-19 and Pdx-1 expression were detected by immunohistochemistry to identify the proliferative characteristics of PSC.Different concentrations of bFGF were used to study the effect on grow curve of PSCs.Results The purified primary-cultured PSCs were obtained after repeated adherence.Immunohistochemistry results showed positive expression of CK-19,and negative expression of Pdx-1 in PSCs.The PSC number of control group,being cultured with no bFGF and of the experimental groups,being cultured with(5 ng/ml),(10 ng/ml) and(20 ng/ml) bFGF was 3.29×10~5/ml,3.47×10~5/ml,3.83×10~5/ml and 3.83×10~5/ml respectively.The PSC numbers of group cultured with(10 ng/ml) and(20 ng/ml) bFGF were significantly higher than that of(5 ng/ml) bFGF group(P<0.01).The cell numbers of all three bFGF groups were significantly higher than that of control group(no bFGF)(P<0.01).Conclusion All three different bFGF concentrations((5 ng/ml),(10 ng/ml) and(20 ng/ml)) can significantly stimulate the proliferation of PSC.更多还原