【摘要】 目的吸烟是增加慢性牙周炎发病率的危险因素,本试验研究体外培养正常人和重度牙周炎患者外周血单个核细胞(PBMC)在尼古丁作用下的白细胞介素-1β(IL-1β)分泌情况。方法采集20例重度牙周炎患者和20例健康志愿者的PBMC,体外培养并与尼古丁(浓度分别为5,25,50,100和200 ng/ml)作用,分别在培养1,2,3,4,5 d后收集上清液,用ELISA法检测上清液中IL-1β的水平。结果5 ng/ml尼古丁对PBMC分泌IL-1β几乎没有影响,与对照组比较差异无显著性(P>0.05),随着尼古丁浓度增加,IL-1β分泌明显增加(P<0.001),并呈浓度时间依赖关系,但当尼古丁浓度超过100 ng/ml,PBMC分泌IL-1β的水平随浓度的增加而下降,与50 ng/ml时比较,差异有显著性(P<0.01)。牙周炎患者PBMC培养3 d后,IL-1β含量为(31.2±7.8)pg/ml,与对照组(23.6±8.1)pg/ml比较差异有显著性(P<0.01);50 ng/ml尼古丁时,牙周炎患者IL-1β为(197.9±26.5)pg/ml,明显高于对照组(117.4±16.8)pg/ml,差异有显著性(P<0.001)。结论尼古丁可致PBMC分泌IL-1β增加,在一定浓度范围内呈时间剂量依赖关系,但高浓度却抑制其分泌;重度牙周炎对尼古丁的刺激更加敏感,提示IL-1β的变化可能是吸烟影响牙周病的机制之一。更多还原

【Abstract】 Objective To observe the effect of nicotine on the secretion of interleukin-1 beta from peripheral blood mononuclear cells(PBMC) in vitro.Methods Twenty non-smoking advaned periodontitis patients and twenty healthy adult volunteers were studied.PBMC were isolated from each blood sample by centrifugation.PBMC(2×10~5 cells/200 microl) were cultured for 1,2,3,5 days in supplemented RPMI1640 alone(control),or in supplemented RPMI1640 containing nicotine(5,25,50,100 and(200 ng/ml)).Culture supernatants were measured for interleukin-1 beta(IL-1 beta) by ELISA.Data was analyzed by ANOVA.Results(5 ng/ml) nicotine had no effect on IL-1 beta secertion by PBMC(P>0.05).Enhanced IL-1 beta secertion was seen when PBMCs were treated with(25 ng/ml) or(50 ng/ml) nicotine in a dose-and time-dependent manner(P<0.001).In contrast,(100 ng/ml) or(200 ng/ml) nicotine significantly downregulated IL-1 beta secretion by human PBMC compared with(50 ng/ml) group(P<0.01 or P<0.001).Advanced periodontitis patients had significantly increased secretion of(IL-1) beta from PBMC treated with(50 ng/ml) nicotine than healthy adult volunteers(P<0.001).Conclusion Nicotin can stimulate PBMC to secrete IL-1 beta in a dose-and time-dependent way,but a great dose of nicotine will inhibit the production of IL-1 beta.These data suggest a potential role of nicotine in pathogenesis of periodontal disease.更多还原