【摘要】 目的建立全血中37种常见药(毒)物的固相萃取-高效液相色谱(SPE-HPLC)分析方法。方法以多沙普仑为内标,0.5mL全血经Oasis小柱固相萃取后,用HPLC进行分析,内源性物质不干扰测定。色谱柱采用LiChrospher!100RP-C18柱(250mm×4.0mm×5μm);二极管阵列检测器,检测波长为230nm和250nm,同时进行紫外扫描;柱温50℃。结果37种药(毒)物的绝对回收率除吗啡外均大于61.68%;日内及日间精密度均小于10%;检测限1～30ng/mL;线性相关系数在0.99798以上。结论本法快速、灵敏、重现性好,可用于实际案例中多种药(毒)物的分析。更多还原

【Abstract】 Objective To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood. Methods With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher?誖 100 RP-C18 (250 mm×4.0 mm×5 μm)column kept at 50 ℃, the DAD detector was set at 230 nm and 250 nm. Results The limit of detection were 1～30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was≥0.997 98. The relative standard deviation for between-day and within-day assay were <10%. Conclusion The method is effective, simple, reliable and has been used in real cases.更多还原