节点文献
陈旧骨骼DNA提取
DNA Extraction from Old Bones
【摘要】 目的寻找从陈旧骨骼中提取DNA的有效方法。方法运用传统的有机法结合Microcon100纯化柱提取骨骼DNA。结果用常规荧光标记复合STR基因分型法可对提取到的陈旧骨骼DNA进行成功分析。结论有机法结合Micrcon100纯化柱提取陈旧骨骼DNA法可有效应用于实际检案。
【Abstract】 Objective To explore an efficient method for extracting DNA from old bones. Methods Using an organic combined with and Microcon 100 to extract and purify DNA. Results The extracted DNA was successfully genotyped by using florescence labeling STR multiplex amplification. Conclusion This method will be useful for forensic scientists in identification of DNA from old bones.
【关键词】 有机法;
Microcon100;
DNA提取;
陈旧骨骼;
【Key words】 organic method; microcon100; DNA extraction; old bones;
【Key words】 organic method; microcon100; DNA extraction; old bones;
- 【文献出处】 法医学杂志 ,Journal of Forensic Medicine , 编辑部邮箱 ,2006年01期
- 【分类号】D919
- 【被引频次】11
- 【下载频次】302