【摘要】 研究毛细血管扩张性共济失调症(AT)患者皮肤的成纤维细胞系AT5BIVA(AT细胞)的应对电离辐射导致细胞氧化损伤的能力。以源于正常人皮肤的成纤维细胞系GM063(GM细胞)为对照,采用超氧化物歧化酶(Superoxide dismutase,SOD)、丙二醛(Malondialdehyde,MDA)测定试剂盒,AT细胞和GM细胞经60Coγ射线0、1、2、3、4Gy照射后,观察比较它们的SOD活性和MDA含量的差异,并分别进行曲线拟合。结果显示在1、2、3、4Gy剂量照射下,AT和GM细胞中SOD活性均随受照剂量增加而明显降低(p<0.01),同一剂量点的AT细胞中SOD活性明显低于GM细胞(p<0.05);AT和GM细胞中MDA含量均随受照剂量增加而升高(p<0.05),同一剂量点的AT细胞中MDA含量明显高于GM细胞(p<0.05)。AT细胞和GM细胞的SOD活性和MDA含量均与照射剂量呈线性关系。结果表明由于AT细胞中SOD活性降低,导致AT细胞应对辐射的氧化损伤能力不足,引起AT细胞中MDA含量增加,是AT细胞高辐射敏感性的原因之一。更多还原

【Abstract】 High radiosensitivity of fibroblast cells (AT5BIVA cells) from skin of Ataxia-telangiectasia (A-T) pa-tients were studied by comparing with GM0639 (GM cells) from the skin of healthy adult. Using SOD (superoxide dismutase) and MDA (malondialdehyde) kit, the activity of SOD and content of MDA in AT and GM cells irradiated to 0, 1, 2, 3, 4Gy by 60Co γ-rays were detected respectively. The dose-response curves of SOD and MDA in AT and GM cells were established. In the irradiated cells, the SOD activity of AT cells were significantly lower than that of GM cells (p<0.05), while the content of MDA of AT cells were significantly higher than that of GM cells (p<0.05). In the two cell types, the activity of SOD had a negative correlation with the irradiation dose (Y=a-bX), whereas the content of MDA had a positive correlation with irradiation dose (Y=a+bX). The results show that the faculty of AT cells to cope with radiation-induced oxidative damage is significantly weaker than that of GM cells.更多还原