【摘要】 为评估低剂量辐射对健康机体产生的生物学风险,检测了低剂量X射线照射对BALB/C小鼠免疫系统的影响。采用X射线全身照射,剂量分别为0、0.39、0.55和1Gy,剂量率为0.2Gy/min,照射24h后取血及器官,用流式细胞仪检测免疫细胞周期和凋亡的变化,用重量法得到胸腺和脾脏指数。发现照射后小鼠的外周血淋巴细胞、胸腺细胞和脾脏淋巴细胞的周期被阻滞在G0—G1期;外周血淋巴细胞和胸腺细胞的凋亡比例随剂量的增大而增大,但是脾脏淋巴细胞的凋亡随着剂量的增大而减小;胸腺和脾脏指数随着剂量的增大而减小。故低剂量X射线全身照射可引起试验动物的免疫细胞周期和凋亡比例发生变化,对其造成一定的损伤;对小鼠免疫系统的影响因器官而异。本实验可为辐射防护提供实验依据。更多还原

【Abstract】 For estimating biological risks from low doses irradiation, BALB/C mice were irradiated to 0, 0.39, 0.55 and 1Gy by X-rays at a dose rate of 0.2Gy/min. The immunocyte cycle and apoptosis were determined by flow cytometry, and the thymus and spleen weight were measured, too. The results showed that the cycle of peripheral blood lymphocyte, thymocyte and spleen lymphocyte were arrested in G0—G1. The apoptosis (expect for spleen lymphocyte) induced by the irradiation increased, whereas the thymus and spleen index declined with increasing dose. The results suggested that low doses X-ray total-body irradiated could result in changes of cycle and apoptosis, and some damages to immunocytes, and the X-ray irradiation affected the organs differently. It might provide experiment basis for radioprotection.更多还原