【摘要】 用高效液相色谱法建立唐古特大黄乙醇提取物的指纹图谱分析方法。采用Phenomenex Kromasil C18色谱柱(4.6 mm×250 mm,5μm);甲醇-0.1%H3PO4为流动相,梯度洗脱,检测波长为270 nm,以大黄酸为参照峰。结果共有18个共有峰。此法为有效地控制唐古特大黄乙醇提取物的质量提供了依据。更多还原

【Abstract】 Fingerprint chromatograms of alcohol extract from Rheum tanguticum by HPLC were established.A Phenomenex Kromasil C₁8（4.6 mm×250 mm,5 μm） column was used,methanol-0.1% phosphate acid was used as the mobile phase,and gradient elution program was used.UV detection wavelength was 270 nm,and rhein was used as a reference peak.The results indicated that 18 peaks were obtained in fingerprint chromatograms.The method is simple and accurate with a good reproducibility,and it can be used as a quality control method for alcohol extracts from Rheum tanguticum.更多还原