【摘要】 建立了比值导数荧光光谱法同时测定色氨酸和5-羟基色氨酸的方法。在比值导数荧光光谱法中,色氨酸浓度在4.0×10-6~2.0×10-5mol/L范围内比值导数荧光光谱峰高与其浓度成正比,线性相关系数为0.9901,检出限为1.3×10-7mol/L。5-羟基色氨酸浓度在4.0×10-8~2.0×10-5mol/L范围内比值导数光谱峰高与其浓度成正比,线性相关系数为0.9996,检出限为1.3×10-8mol/L。同时测定了实际样品中的色氨酸和5-羟基色氨酸,测定结果与高效液相色谱法有良好的一致性。更多还原

【Abstract】 First-order derivative ratio fluorescence spectrometry（DRFS） for the simultaneous determination of tryptophan and 5-hydroxytryptophan are developed.In the DRFS method,the peak heights of derivative ratio spectra at 312 nm are proportional to the concentration of tryptophan and 5-hydroxytryptophan with linear correlation coefficients of 0.9901 and 0.9996,respectively.Their detection limits are 1.3×10^-7 mol/L and 1.3×10^-8 mol/L,respectively.Compared with the results for practical samples obtained by HPLC,the two methods show a good correspondance.更多还原