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芹菜中黄酮类物质的提取和测定

Extraction and determination of flavonids in celery

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【作者】 何书美刘敬兰

【Author】 HE Shu-mei~ and LIU Jing-lan(Hebei normal university Experiment central,Shijiazhuang 050016)

【机构】 河北师范大学实验中心河北师范大学实验中心 石家庄050016石家庄050016

【摘要】 采用碱溶酸析法,成功地提取了芹菜中黄酮类物质。经HCl-镁粉反应和红外光谱鉴定,提取物为黄酮类物质。另外,利用分光光度法,以芦丁为标准对芹菜的叶和茎中的总黄酮分别进行了测定,其质量分数分别为10.19 mg/g,3.064 mg/g。同时讨论了时间及色素对测定方法的影响,结果表明,显色时间在40 min内稳定,色素引起误差为5%。

【Abstract】 In this paper,flavonids were extracted from celery successfully,by using the method of "dissolved in alkali and separated out in acid".Flavonids were identified by reacting with magnesium-hydrochloric acid and IR characterization.In addition,all the flavonids in leaves and culms of celery were determined by spectrophotometry with rutin as the standard,and the results were 10.19% and 3.064%,respectively.Effects of time and pigment on the determination results were investigated,and the experimental results indicated that the absorbency was stable within 40 min and the error from pigment was 5%.

【关键词】 黄酮类化合物芹菜提取测定
【Key words】 FlavonidsExtractionDeterminationCelery
【基金】 河北师范大学自然科学基金(2005Y13)
  • 【文献出处】 分析试验室 ,Chinese Journal of Analysis Laboratory , 编辑部邮箱 ,2006年08期
  • 【分类号】TS255.7
  • 【被引频次】29
  • 【下载频次】916
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