【摘要】 合成了甲福明的分子印迹聚合物,以此聚合物为识别物质,在线分离富集甲福明,建立了一种测定甲福明的流动式化学发光但感器。N-溴代丁二酰亚胺(NBS)和荧光素与甲福明发生化学反应,产生强的化学发光。甲福明质量浓度在2×10-8~8×10-6g/mL范围内同发光强度成良好线性关系,方法的检出限为6×10-9g/mL,相对标准偏差小于5%(n=9)。选择性实验表明将分子印迹聚合物作为识别物质应用于化学发光分析中,能大大提高化学发光分析方法的选择性。该传感器可逆性强、稳定性好,可重复使用100次以上,已用于人体尿样中甲福明的测定。更多还原

【Abstract】 Molecularly imprinted polymer of metformin was synthesized and a chemiluminescence sensor was prepared using the polymer as recognition material for the determination of metformin.NBS and fluorescein reacted with metformin to produce strong CL.The CL intensity was linear to metformin concentration in the range from 2×10^-8 to 8×10^-6 g/mL.The detection limit was 6×10^-9 g/mL and the R.S.D.was less than 5%（n=7）.The selective experiment showed that it could greatly improve the selectivity of CL method when MIP was applied to CL.The sensor was reversible and could be used for more than 100 times.It has been used to determine the metfomin in human urine samples.更多还原