【摘要】 己二酸是一种重要有机化合物,具有强极性和离子性的特点,由于在210 nm以上有弱的紫外吸收,故用气相色谱(GC)或高效液相色谱(HPLC)直接分离测定均有一定困难。为此,本文从研究乙酸缓冲溶液中己二酸-铜络合物的紫外吸收光谱出发,建立了高效液相色谱紫外检测己二酸的方法。通过实验确定了最优色谱条件。本法测定己二酸的线性相关系数为0.998,回收率为97.5%,检出限为0.32 mg/mL。此方法可用于监测生物催化法生产的己二酸,优化生产条件。更多还原

【Abstract】 Biocatalysis is a new way used in chemical field.Biological processes are commonly perceived as being less harmful to environment than chemical manufacturing processes.To analyze adipic-acid produced by biocatalysis,adipic acid-copper complex was analyzed by high performance liquid chromatography with UV-detector.The effects of mobile phase such as the concentration of methyl alcohol,copper ions and buffer as well as pH values were discussed.Guidelines were given for optimization of the separation.The separation was carried out on a C18 column with the flow rate of mobile phase of 0.6 mL/min and the detection wavelength of 254 nm.更多还原