【摘要】 以宽谱带高压汞灯为光源的倒置荧光显微镜,配备电荷耦合器件(CCD)及微流控芯片,自组装芯片毛细管电泳荧光电荷耦合器件检测系统。选择不同激发波长(340~540 nm)荧光试剂,进行荧光检测,荧光素的检出限(S/N=3)为7.3×10-9mol/L,荧光素异硫氰酸酯(FITC)的检出限(S/N=3)为1.7×10-8mol/L,显示了该系统对荧光试剂选择范围宽、灵敏度高的特点。用该系统成功分离了FITC与荧光素、曙红与荧光素两种混合荧光试剂。实验表明,组装的芯片毛细管电泳CCD荧光检测系统是成功的。更多还原

【Abstract】 A detection system in microchip capillary electrophoresis has been developed in this paper,mainly including an inverted fluorescence microscope equipped with a 100 W high-pressure mercury lamp and CCD.This system could be applied to the detection of various wavelength fluorescence（340⁵40 nm）.The detection limit（S/N=3） of 7.3×10^-9mol/L and1.7×10^-8mol/L for fluorescein and FITC were obtained,respectively.We have successfully separated FITC-fluorescein and eosin-fluorescein two mixed fluorescent substances.It proved that this fluorescent detection system could be successfully applied to microchip electrophoresis.更多还原