【摘要】 利用高效液相色谱与电喷雾质谱联用技术研究了枳壳中的黄酮苷类化合物。实验采用反相C18色谱柱,二元线性梯度洗脱,分离并检测了枳壳中的6种黄酮苷类化合物;它们分别是新圣草苷(neoeriocitrin)、异柚皮苷(isonaringin)、柚皮苷(naringin)、橙皮苷(hesperidin)、新橙皮苷(neohesperidin)和新枸橘苷(neopon-cirin);通过与电喷雾质谱联用获得了这6种黄酮苷的准分子离子峰(M+H+)及分子加钠峰(M+Na+),利用质谱的碰撞诱导解离技术获得了碎片裂解信息。通过这此质谱信息并结合文献,对这6种化合物进行了结构鉴定。更多还原

【Abstract】 A high performance liquid chromatography-electrospray ionization mass spectrometric method was developed for analysis and identification of flavonoid glycosides extracts from Fructus aurantii.Samples were analyzed by a reversed-phase C_ 18 column using a binary eluent under gradient conditions. Six flavonoid glycosides could be separated and detected. The [M+Na]~ + and [M+H]~ + ions of flavonoid glycosides in the positive mode were observed for molecular mass information. A collision induced dissociation(CID )experiment was carried out in order to identify the structures of flavonoid glycosides.更多还原