【摘要】 用高效液相色谱-二极管阵列检测器(HPLC-PDA)建立了烟草中绿原酸、莨菪亭、芸香苷同时测定的方法。烟草样品用甲醇/水(4∶1,V/V)超声萃取,过滤,上样。用Hypersil ODS(4.6 mm(i.d.)×150 mm,3μm)色谱柱,甲醇/TFA水溶液梯度洗脱,PDA光谱图辅助定性,内标法定量。该方法预处理简单,重现性好,回收率84.4%~96.4%;相对标准偏差为1.4%~6.4%。更多还原

【Abstract】 Chlorogenic acid,chrysatropic acid and rutin in tobacco were analyzed using high performance liquid chromatography(HPLC)/photodiode array deteciton(PDA).After the tobacco was simply treated by ultrasonic extraction using methanol/water(4∶1,V/V) and filtration,the obtained liquid sample was analyzed by HPLC(using) hypersil ODS column(4.6 mm×150 mm i.d.,3 μm).Methanol/trifluoroacetic acid(TFA) aqueous solution was used as mobile phase for gradient elution.PDA spectrum was used for assistant identification.Internal standard method was applied for quantitation.The recovery was in the range of 84.4%-96.4%.The relative standard deviation of determination was 1.4%-6.4% for three compounds.更多还原