【摘要】 采用一种新型紫外衍生试剂对乙酰氨基苯磺酰氟(PAABS-F)作为柱前衍生试剂,成功地对19种标准D-氨基酸和甘氨酸进行了衍生和毛细管电泳分离。详细研究了各种分离条件对毛细管电泳分离的影响。实验结果表明:采用20 mmol/L硼砂(pH 9.3),126 mmol/L十二烷基硫酸钠,8 mmol/Lβ-环糊精和20mmol/L NaC l时分离结果最佳,16 m in内实现了20种氨基酸的基线分离。更多还原

【Abstract】 A new method for the separation of 19 standard D-amino acids and glycine(Gly) by capillary electrophoresis(CE) using p-acetamidobenzenesulfonyl fluoride(PAABS-F) as precolumn derivatization reagent has been developed.The separation conditions were carefully studied and optimized. The experimental results showed that 20 mmmol/L sodium borate(pH 9.3),126 mmmol/L sodium dodecyl sulfate,8 mmol/L(β-cyclodextrin) and 20 mmol/L NaCl were the optimal conditions for the CE separation.Under the optimal(conditions),19 stangdard D-amino acids and Gly were successfully achied with baseline separation within(16 min) by CE.更多还原