【摘要】 在pH 4.56的B ritton-Rob inson(B-R)缓冲溶液中,半胱氨酸的SH和NH3+分别与金纳米粒子表面进行共价结合和静电作用,导致金纳米粒子的长距离自组装,形成网状超分子结构,并使金纳米粒子的最大吸收波长从520 nm红移到660 nm。本实验对半胱氨酸引导的金纳米粒子自组装的作用机制进行了研究,建立了操作简便、高灵敏度测定半胱氨酸的分析方法。其线性范围为0.01~0.20 mg/L;检出限为2.8μg/L(3,σ2.3×10-8mol/L)。在实验条件下,其它常见的氨基酸和谷胱甘肽均不干扰测定。更多还原

【Abstract】 Due to the covalent combination with the SH group and the electrostatic binding with the NH⁺₃ group of cysteine,gold nanoparticles can self-assemble and form a supermolecular network structure in the（Britton-Robinson） buffer solution（pH 4.56）.As a result,absorption peak of the gold nanoparticles shifts from 520 nm to 660 nm.The mechanism of self-assembly of gold nanoparticles directed by cysteine was（investigated）.Based on the study,a simple and sensitive method for cysteine determination has been established.There is a good linear relationship between the absorbance at 660 nm and cysteine concentration in the range of 0.01-0.20 mg/L.The corresponding detection limit is 2.8 μg/L(3σ,2.3×10^-8（mol/L）).（Common） amino acids and glutathione do not interfere with the determination.更多还原