【摘要】 使用新型的亲水性较强的C16硅胶反相色谱柱,以水/磷酸缓冲液/乙腈为流动相,流速1mL/m in,在17m in内分离了头孢羟氨苄、头孢克洛、头孢氨苄、头孢拉定和头孢噻吩5种头孢类抗生素。分离后的化合物在紫外检测器上检测,检测波长270 nm,线性范围0.1~40 mg/L(r≥0.9993),5种化合物的检出限依次为9.7、6.9、5.6、6.4和4.9μg/L。该方法成功的应用于人尿液和牛奶中头孢类抗生素的检测,2 mg/L浓度水平的加标回收率在96.5%~105%之间。更多还原

【Abstract】 A simple method for the separation and determination of cefadroxil,cefaclor,cefaloxin,cefradine and cefalotin etc.was completed on a C₁₆ silica stationary phase packed reversed-phase column with mobile phase of water/acetonitrile/phosphoric buffer（1 mL/min） in about 17 min.The compounds were detected（directly） by ultraviolet detector at 270 nm.The linear range was 0.1-40 mg/L（r≥0.9993） and the detection limits of cefadroxil,cefaclor,cefaloxin,cefradine and cefalotin were 9.7,6.9,5.6,6.4 and 4.9 μg/L,（respectively）.The method was applied successfully to the detection of cephalosporins in human（urine） and in（bovine） milk with the recovery of 96.5%-105%（when added sample was 2 mg/L）.更多还原