【摘要】 采用胶束电动毛细管电泳(MEKC)法测定多种β-内酰胺类抗生素及舒巴坦的含量。电泳运行缓冲液为20 mmol/L硼砂缓冲液(pH9.5),十二烷基硫酸钠(SDS)38 mmol/L。8种药物在15m in内达到基线分离。选择苯巴比妥作为内标,各组分浓度在50~600 mg/L范围内呈良好线性关系,相关系数为0.9932~0.9993;检出限4.8~26 mg/L;进样精密度RSD 3.0%~5.6%;对头孢哌酮-舒巴坦制剂进行含量测定,回收率为100%~106%,含量测定结果与HPLC方法所得结果没有显著性差异。更多还原

【Abstract】 The application of micellar electrokinetic capillary chromatography for separation and determination antibiotics of β-lactam and β-lactamase inhibitor was investigated.20 mmol/L sodium tetraborate buffer(using sodium hydroxide adjusted to pH 9.5) and 38 mmol/L sodium dodecyl sulfate(SDS) and electrophoresis(voltage) of 20 kV seem to provide optimal conditions for the separation and determination.Eight analytes reach the baseline separation in 15 minutes.The concentration ranges of the linearity were all about 50～600 mg/L,and the correlation coefficient is between 0.9932 and 0.9993.The relative standard deviation of 8 analytes ranges from 3.0% to 5.6%,with the limit of detection(S/N=3) 4.8~26 mg/L.The recovery is between 100% and 106% for the determination of cefoperazone-sulbactam preparation.Statistical analysis by t-test showed no significant differences between the results obtained by micellar electrokinetic capillary chromatography and by high performance liquid chromatography.更多还原