【摘要】 目的:探讨微流体芯片检测乙型肝炎病毒基因型的价值。方法:应用微流体芯片及基因测序技术检测41例慢性乙型肝炎患者感染的乙型肝炎病毒基因型;比较两种方法检测结果的一致性。结果:41例HBV患者中B基因型21例(51.2%),C基因型20例(48.8%),未检测到B+C混合基因型感染。微流体芯片检测结果与基因测序结果完全一致。结论:微流体芯片技术可以准确快速检测乙型肝炎病毒基因型,对乙型肝炎病毒的分子流行病学研究具有重要意义。更多还原

【Abstract】 Objective To evaluate the applicability of micro-fluid chip in genotyping of hepatitis B virus.Methods 41 samples were examined with both micro-fluid chip genotypeing and DNA sequence analysis and the results were compared.Results In 41 samples,21 samples were classified as genotype B(51.2%),20 samples were classified as genotype C(48.8%) with no mixed genotype of B and C.The results with both methods were entirely similar.Conclusion The genotype of hepatitis B virus may be accurately identified with micro-fluid chip.The technology involved is much simpler than that with either monoclonal antibody ELISA or PCR and is most applicable for massive amount of specimens.更多还原