【摘要】 目的研究系统性红斑狼疮（SLE）患者外周血CD4⁺、CD25⁺、CD4⁺CD25⁺淋巴细胞亚群的变化及临床意义。方法采用二色荧光抗体标记法,以流式细胞仪对34例SLE患者和18名正常人外周血CD4⁺、CD25⁺、CD4⁺CD25⁺淋巴细胞亚群进行了检测,以CD25抗原荧光强度≥10的细胞定义为CD25^high细胞,分析其百分率和荧光强度,并结合临床资料进行相关分析。结果活动组SLE患者外周血CD4⁺CD25⁺细胞、CD4⁺CD25^high细胞占总淋巴细胞的百分率[（4．80±1．21）%和（0．25±0．10）%]均低于正常对照组[（8．92±3．21）%和（0．44±0．22）%],亦低于稳定组SLE患者[（11．28±2．09）%和（0．59±0．34）%]（P均＜0．05）,而稳定组SLE患者与正常对照组差异无统计学意义（P＞0．05）；但CD4⁺CD25⁺细胞占CD4⁺细胞的比例在三组之间差异无统计学意义（P均＞0．05）；外周血CD4⁺CD25⁺、CD4⁺CD25h^high细胞数与系统性红斑狼疮疾病活动指数（SLEDAI）积分呈负相关（r=-0．74,P=0．004和r=-0．614,P=0．026）,与其他临床指标如补体、抗核抗体（ANA）滴度等无相关性；活动组SLE患者外周血CD4⁺和CD25⁺细胞亦均低于正常对照组[（23±7）vs（34±7）和（7．4±1．8）vs（13．9±3．4）,P＜0．05]；SLE患者CD25抗原的荧光强度高于正常对照组（P＜0．05）,而CD4抗原的荧光强度差异无统计学意义（P＞0．05）。结论活动性SLE患者外周血CD4⁺ CD25⁺细胞数是减少的,并与CD4⁺细胞的减少有关,提示在SLE的发病及病情活动中可能起一定作用。更多还原

【Abstract】 Objective To investigate the expression and clinical significance of peripheral blood CD4⁺, CD25⁺ and CD4⁺CD25⁺ T subpopulations in patients with systemic lupus erythematosis.Methods The per- centage and fluorescence intensities of peripheral blood CD4⁺,CD25⁺ and CD4⁺CD25⁺ subpopulations from 34 SLE and 18 normal controls were measured with flow cytometry assay,then the correlation with clincal data was analyzed.The CD25⁺ cells were defined as the CD25^high cells if their fluorescence intensity was higher than 10. Results The percentage of CD4⁺CD25⁺,CD4⁺CD25^high T lymphocytes in active SLE patients[（4.80±1.21）% and （0.25±0.10）%]was lower than that in normal controls[（8.92±3.21）% and（0.44±0.22）% and non-active SLE patients（11.28±2.09）% and（0.59±0.34）%]（P＜0.05）.However,as for the CD25⁺ cells in the CD4⁺ T cells,there was no difference between SLE patients and normal control group.Peripheral blood CD4⁺CD25⁺,CD4⁺CD25^high cells in SLE were reversely correlated with SLEDAI（r=-0.74,P=0.004 and r=-0.614,P=0.026）,but not with others such as complements,ANA titers etc.Peripheral blood CD4⁺ and CD25⁺ lymphocytes in active SLE pa- tients were also lower than those in normal controls[（23±7）vs（34±7）and（7.4±1.8）vs（13.9±3.4）,P＜0.05]. CD25 fluorescence intensities were higher in the SLE patients those in the normal controls,but CD4 fluores- cence intensities were not.Conclusion CD4⁺CD25⁺ may play a role in the pathogenesis of SLE.更多还原