【摘要】 目的观察地塞米松对骨髓基质细胞（MSCs）增殖及成骨分化的影响。方法以离心法分离培养MSCs,分别以10^-9、10^-8和10^-7 mol/L地塞米松对细胞进行干预,采用四甲基偶氮唑蓝比色法（MTT法）测细胞增殖率；测细胞质碱性磷酸酶（ALP）含量；利用反转录聚合酶链反应（RT-PCR）技术在转录水平检测成骨细胞标记物骨桥蛋白（OPN）mRNA的表达。结果地塞米松干预8 d后,各干预组的吸光度值均低于对照组,10^-8和10^-7 mol/L地塞米松组与对照组相比差异有统计学意义（P＜0．05）；地塞米松干预12 d后,各干预组细胞质ALP含量呈浓度依赖性增加,与对照组相比差异均有统计学意义（P＜0．05）；地塞米松干预12 d后,各干预组均有OPN mRNA表达,且随浓度增加表达增强。结论地塞米松对体外MSCs增殖有抑制作用,但对MSCs成骨分化有促进作用。更多还原

【Abstract】 Objective To observe the effect of dexamethasone（Dex）on the proliferation and os- teogenic differentiation of human marrow stromal cells（MSCs）in vitro.Methods The primary human MSCs were isolated and cultured by Ficoll seperation culture in vitro.In subcultures,human MSCs were respectively treated with dexamethasone 10^-9,10^-8 and 10^-7 mol/L.The proliferation of human MSCs was measured using MTF method;cytoplasmic alkaline phosphatase（ALP）activity was measured;the osteogenic marker osteopontin （OPN）mRNA were examined by reverse transcriptase polymerase chain reaction（RT-PCR）.Results The op- tical density values in cultures treated with dexamethasone 10^-8 and 10^-7 mol/L for 8 days were significantly lower than those in the controls（P＜0.05）.Treatment of cells with Dex for 12 days led to a significant increase in cytoplasmic ALP activity（P＜0.05）in a dose-dependent manner.Dex induced OPN mRNA.Conclusion Dex inhibits the proliferation of human MSCs and dexamethasone 10^-7 mol/L leads to a strong decrease in cell number.Dex induces human MSCs differentiate to osteoblastic cells.更多还原