【摘要】 目的通过观察p27kip1、ki-67的表达探讨子宫内膜细胞增殖能力对子宫内膜异位症发病的影响。方法应用免疫组化SP法检测36例子宫内膜异位症患者(EMs组)的异位和在位内膜及23例正常子宫内膜中p27kip1、ki-67的表达情况。结果①异位内膜p27kip1腺体胞质表达率较正常子宫内膜明显增强(P<0·05);异位内膜p27kip1腺体胞核表达率较正常子宫内膜明显下降(P<0·05)。②正常子宫内膜与在位内膜腺体ki-67表达增生期显著高于分泌期(P<0·05);异位内膜ki-67表达无周期性变化;异位内膜ki-67分泌期增殖指数显著高于在位内膜(P<0·01)。③EMs组异位内膜p27kip1与ki-67表达呈负相关(P<0·05)。结论ki-67增殖指数反映了子宫内膜细胞的增殖活性,异位内膜细胞增殖活跃,而p27kip1表达下降,负性调节细胞增殖能力下调,p27kip1和ki-67可能在子宫内膜异位症发生发展中起重要作用。更多还原

【Abstract】 Objective To investigate the expression of the cell cycle inhibitor p27 kip1 and cell proliferation associated nuclear antigen ki-67 in the pathogenesis of endometriosis.Methods Expressions of p27 kip1 and ki-67 were detected in the eutopic and ectopic endometrium from 36 patients with endometriosis and 20 normal endometrium from healthy women immunohistochemically.Results ① The nuclear expression of p27 kip1 in ectopic endometrium was significantly less than that in normal endometrium (P<0.05), and in plasma p27 kip1 expression was more in ectopic endometrium (P<0.05).② The ki-67 expression in glandular cells of the normal endometriosis and eutopic endometrium in the proliferative phase were more than that in the secretory phase (P<0.01).Ectopic endometrium showed no cyclic change.The expression rate of ki-67 in ectopic endometrium was significantly higher than that in normal endometrium and eutopic endometrium (P<0.01).③ The expression of p27 kip1 was negatively related to ki-67 (P<0.05). Conclusion The expression of the cyclin kinase inhibitor p27 Kip1 and ki-67 play important role in pathogenesis of endometriosis.更多还原