【摘要】 目的探讨末端支链脂肪酸13-甲基肉豆蔻酸(13-MTD)抗小鼠肿瘤作用及其对肉毒碱棕榈酰转移酶(CPT-1)活性影响的相关性。方法荷U14肿瘤的ICR小鼠分5组:阴性对照组给水,阳性对照组以环磷酰胺灌胃,13-MTD组分为高、中、低剂量组,每日灌胃,共12 d,取瘤称质量,计算抑瘤率。从各给药组瘤组织提取线粒体,测定各个肿瘤线粒体的CPT-1活性,计算相应肿瘤质量与CPT-1酶活性的相关系数。试管内测定13-MTD对提取线粒体的CPT-1的直接作用。结果13-MTD对U14肿瘤的抑制作用表现为剂量依赖性,高剂量抑制率达64.9%。瘤质量与CPT-1酶活性的相关系数r=0.584,P<0.05。在试管内13-MTD对U14肿瘤的CPT-1酶活性呈剂量依赖的直接抑制作用。结论13-MTD能直接抑制U14肿瘤CPT-1酶,抗肿瘤作用与抑制肿瘤线粒体CPT-1酶活性正相关。更多还原

【Abstract】 Objective To investigate anticancer effects of a branched-chain fatty acid,13-methyltetradecanoic acid(13-MTD),and the relationship between the anticancer effects and its inhibition of mitochondria carnitine palmitoyltransferase 1(CPT-1) activity.Methods ICR mice bearing cervical tumor U14 were treated with varied dosage of 13-MTD for 12 d,at the end of experiment,the mice were killed and the tumors were weighted.CPT-1 activity was measured with the mitochondria isolated from the 13-MTD treated tumors,and the correlation between the CPT-1 activities and the related tumor weight was determined.(Results) 13-MTD significantly inhibited the growth of tumor U14,with a maximum inhibition rate of 64.9%.The correlation between the CPT-1 activity and tumor weight index was r=0.584(n=20,P<0.05).Conclusion 13-MTD can inhibit the growth of murine cervical tumor U14 and inhibit the tumor mitochondria CPT-1 activity in vitro.Anticancer effect of 13-MTD ascribed to the inhibition of CPT-1 activity.更多还原