【摘要】 采用农杆菌介导的方法将高赖氨酸蛋白基因导入台粳9号幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得2株转基因植株.对这些植株及后代植株进行GUS染色和PCR及PCR Southern b lot检测分析,确定高赖氨酸蛋白基因已整合到台粳9号基因组中,并能稳定遗传表达.经检测,转基因水稻糙米赖氨酸含量为0.349%,比对照提高29.3%;转基因水稻秸秆赖氨酸含量为0.341%,比对照提高8.3%.更多还原

【Abstract】 Lysine-rich protein gene was transformed to the embryonic callus tissue of Taijing 9 with Agrobacterium-mediated method.After resistance screening by hygromix,the resistant calli differentiated to seedlings,and 2 transgenic plants were obtained.PCR testing and GUS staining analysis showed that Lysine-rich protein gene had been transformed to the genome of Taijing 9,and it could be stably expressed in its next generation.The test results showed that lysine content in unpolished grains of transgenic rice was(0.349%,) improved 29.3% compared to control;and lysine content in transgenic rice straw was 0.341%,enhanced 8.3% compared to control.更多还原