【摘要】 采用横切薄片培养方法建立了香蕉基因转化的适宜受体系统.结果表明:在30℃、黑暗培养15 d,而后转入24℃、光照培养5 d的条件下,香蕉横切薄片的出芽率较高,植株生长较健壮,适宜用作转基因的受体;羧苄青霉素对香蕉薄片的生长没有明显的抑制作用,可以作为转化的抑菌剂;香蕉对潮霉素比较敏感,能够显著抑制香蕉横切薄片的出芽率,确定用40mg.L-1作为潮霉素的筛选含量.更多还原

【Abstract】 An appropriate transgenic acceptor system for banana（Musa spp.） was developed in this experiment.The high efficiency of shoot differentiation was obtained from the thin-cross-section cultured at 24 ℃ in light for 5 days after being cultured at 30 ℃ in darkness for 15 days.The regenerated plantlets grew well and the thin-cross-sections of the plantlets could be used as transgenic acceptors for Agrobacteriummediated transformation.Carbenicillin could not inhibit growth and differentiation of the thin-crosssections of banana and could be used to kill Agrobacterium during the selection stage.Banana was sensitive to hygromycin B,and hygromycin B could inhibit growth and differentiation of the thincross-sections of banana,which could be used as a selection agent for banana transformation.The results showed that 40 mg·L^-1 hygromycin was the lowest concentration for the selection.更多还原