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产碱性蛋白酶海洋细菌的筛选与鉴定
Isolation and identification of alkaline protease-producing marine bacteria
【摘要】 从福建海域的海水、海泥及海藻样品中分离到3株产碱性蛋白酶的海洋细菌菌株3B、6CW、15E,采用16S rDNA分子生物学鉴定结合细菌的常规鉴定方法,确认分离到的3株产蛋白酶的海洋细菌菌株分别是荧光假单胞菌(Pseudom onas fluo-rescens)、粘质沙雷菌(Serratia marcescens)和氧化短杆菌(Brevibacterium oxydans),并对其发酵液中的蛋白酶活力做了初步研究.
【Abstract】 Three alkaline protease-producing marine bacterial strains,named as 3B,6CW and 15E,were isolated from samples of sea-water,sea-mud and seeweed.These isolates were characterized and identified as species of Pseudomonas fluorescens, Serratia marcescens,and Brevibacterium oxydans respectively,based on their physiological and biochemical characteristics and analysis of 16S rDNA sequence.And proteolytic activities of protease from strains 3B,6CW and 15E were researched.
【基金】 福建省自然科学基金资助项目(Z0516011);福建省科技计划项目(2003F011)资助
- 【文献出处】 福建农业大学学报 , 编辑部邮箱 ,2006年04期
- 【分类号】Q93-33
- 【被引频次】36
- 【下载频次】727