【摘要】 本试验扩增并克隆测定了牦牛2个α-珠蛋白基因间区域的序列.根据所测结果及人、鼠、山羊、马和黄牛的α-珠蛋白基因序列比对中的保守区设计了2对特异性引物,扩增并克隆测定了牦牛α-珠蛋白的上、下游基因序列.将测定的牦牛α-珠蛋白基因间区域序列进行同源性搜索,发现没有任何一个基因与它同源.上、下游基因的测序结果表明,上游基因为牦牛α1-珠蛋白基因,下游基因为α2-珠蛋白基因.这表明牦牛的2个α-珠蛋白基因在染色体上紧密排列,其中α1-珠蛋白基因在前,α2-珠蛋白基因在后.更多还原

【Abstract】 Two differential primers were designed from the sequence of yak′s two α-globin genes′s intergenic region which was cloned and sequenced,and from the conserved region of human,mouse,goat,horse and cattle′s α-globin gene for confirming the order of yak′s two α-globin genes.There was no other gene in the intergenic region of yak′s two α-globin genes after blasting.The upstream gene was identified as yak′s α₁-globin gene,while the downstream gene was identified as yak′s α₂-globin gene after cloning and sequencing.These indicate that the yak′s two α-globin genes are located in the chromosome tightly,α₁-globin gene in the front and α₂-globin gene at the back.更多还原