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龙眼体细胞胚胎发生过程中内源多胺的变化

The changes of the contents of endogenous polyamines in the process of somatic embryogenesis from embryogenic callus in longan

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【作者】 陈春玲赖钟雄

【Author】 CHEN Chun-ling~(1,2),LAI Zhong-xiong~1(1.Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;2.Department of Plant Tissue Culture,Beijing Botanical Garden,Beijing 100093,China)

【机构】 福建农林大学园艺植物生物工程研究所福建农林大学园艺植物生物工程研究所 福建福州350002北京植物园组培室北京100093福建福州350002

【摘要】 采用高效液相色谱测定了龙眼胚性愈伤组织体胚发生过程中内源多胺含量的变化.结果表明:龙眼胚性愈伤组织中内源多胺含量比非胚性愈伤组织高;体胚发生过程中内源多胺含量变化趋势大致呈“M”状,在胚性愈伤组织Ⅱ和球形胚阶段转折;内源多胺含量的变化先于形态学的变化.

【Abstract】 The changes of the contents of endogenous polyamines of synchronic materials at different developmental stages in somatic embryogenesis were measured by high-performance liquid chromatography(HPLC) in longan.The results showed that the levels of endogenous polyamines in embryogenic calli were higher than those in non-embryogenic calli;the changes of endogenous polyamines in the process of somatic embryogenesis approximately showed an "M" curve during embryogenic callus Ⅱ and globular embryoids;the content of polyamines was the 2 turning-point stages in the changes of endogenous polyamines in somatic embryogenesis;the changes of endogenous polyamines were earlier than those of morphogenesis.

【关键词】 龙眼体胚发生内源多胺
【Key words】 longansomatic embryogenesisendogenous polyamines
【基金】 国家自然科学基金资助项目(30471204);霍英东青年教师基金资助项目(71026);福建省自然科学基金资助项目(C0410012)
  • 【分类号】S667.2
  • 【被引频次】24
  • 【下载频次】200
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