【摘要】 通过提纯中华蜜蜂工蜂体内的碱性磷酸酶,研究金属离子及脲对碱性磷酸酶的影响.结果表明:Na+、K+和L i+等正一价金属离子浓度为1.0-10.0 mmol.L-1时,对酶活力没有影响;Mg2+、Ca2+、Ba2+浓度为0-5.5 mmol.L-1时,对酶均有激活作用;N i2+、Mn2+、Co2+、Zn2+浓度为0-200μmol.L-1时,对酶有激活作用,而Cu2+在该浓度下对酶有抑制作用;Hg2+、Ag+及Cd2+浓度为0-150μmol.L-1时对酶有抑制作用;脲浓度低于3 mol.L-1或高于3 mol.L-1时,对碱性磷酸酶有变性失活作用.更多还原

【Abstract】 The effects of metal ions and urea on the alkaline phosphatase（AKP） from Apis cerana cerana Fabricius were studied.The results were as follows: Na⁺,K⁺ and Li⁺(1.0-10.[KG8]0 mmol·L^-1) had no effect on the enzyme activity;Mg²⁺,Ca²⁺,Ba²⁺(0-5.5 mmol·L^-1) could activate the enzyme;Ni²⁺,Mn²⁺,Co²⁺and Zn²⁺(0-200 μmol·L^-1) could activate the enzyme,but in this range of concentration,Cu²⁺ could inhibit the enzyme;in the range of 0-150 μmol·L^-1,Hg²⁺,Ag⁺ and Cd²⁺ could inhibit the AKP;the concentration of the urea(lower than 3 mol·L^-1 or higher than 3 mol·L^-1) inactivated the enzyme.更多还原