【摘要】 以3年生柳桉优树环割促萌枝条为外植体,以MS为基本培养基,通过激素种类与浓度、大量元素配比等试验,探讨了柳桉腋芽诱导、继代增殖、生根培养基配方。结果表明:外植体宜采用中等幼嫩的茎段,用75%酒精消毒30 s、0.1%升汞溶液消毒4 min效果最佳;在3/4 MS+0.3 mg.L-16-BA+0.2 mg.L-1NAA培养基上进行侧芽诱导,柳桉外植体出芽率最高;在改良MS+0.2 mg.L-16-BA+0.2 mg.L-1NAA继代培养基上,柳桉增殖效果最好,其芽体健壮,生长正常,增殖率3倍以上;应用1/2MS培养基附加ABT 1#0.5 mg.L-1和IBA 0.1 mg.L-1,其生根率可达95%以上,生根3~4条,苗木生长健壮,移栽成活率可达92%以上。这些配方已成功地应用于柳桉优良无性系苗木的工厂化生产,单个超净台月苗木生产能力达7万株以上。更多还原

【Abstract】 A tissue culture protocol by shoot proliferation is developed for Eucalyptus saligna through adjusting the plant growth regulators and ratio of macro-elements using sprouts collected from stubs of three-year-old plus trees as explants.The main results are as follows: 1.Half lignified shoots were most suitable for explants.Dipping them in solution of 75% alcohol for 30 seconds and then in 0.1% HgCl₂ for 4 minutes are the best way for their disinfection;2.3/4 MS supplemented with 0.3 mg·L^-1 6-BA,0.2 mg·L^-1 NAA and 20 g·L^-1 sugar is crucial for shoot germination and proliferation,while amended MS supplemented with 0.2 mg·L^-1 6-BA,0.2 mg·L^-1 NAA and 30 g·L^-1 sugar for clump shoot induction;3.1/2 MS supplemented with 0.5 mg·L^-1 ABT-1 and 0.1 mg·L^-1 IBA is suitable for rooting,with rooting rate up to 95%;and 4.The survival rate of out-planting in nursery is more than 92%.The protocol has been successfully used in large-scale clonal production of the seedlings.更多还原