【摘要】 对桉树组培工厂化育苗培养基的调配进行了系统的研究,以尾巨桉DH32-29优良无性系组培苗为试验材料,探讨了桉树组培工厂化育苗生产中影响的关键因素,结果表明:琼脂成分、激素浓度和种类、大量元素含量对组培苗生长有显著影响。继代培养阶段,培养基中添加琼脂条时变异苗率低且平稳,增殖阶段,培养基中激素6-BA和NAA的浓度分别在0.25~0.30mg.L-1和0.10~0.15 mg.L-1范围内时增殖效率最好。壮苗阶段,培养基中激素6-BA和NAA的浓度分别在0.25~0.27mg.L-1和0.10~0.15 mg.L-1范围内时有效出苗率最佳。生根培养阶段,培养基中添加浓度为40%的大量元素,其生根率达95%,且不影响后续生长,生根激素在继代培养3代以后可降低为ABT 0.5 mg.L-1和NAA为0.5 mg.L-1。更多还原

【Abstract】 Take the tissue culture seedlings of Eucalyptus urophylla×E.grandis DH32-29 clone as experiment material,adjustment technique of culture medium and key factors were studied in industrialization of seedling production of eucalyptus in the paper.The result showed that,the composition of agar,the concentration and kinds of hormone,and the contents of macro-element had distinct influence on the growth of tissue culture seedlings.At the subculture phase,the rate of variation seedlings was very low and stable when agar bars was added in the culture medium;at the proliferative phase,the highest proliferative efficiency was gained when 6-BA(0.25～0.30 mg·L~-1) and NAA(0.10～0.15 mg·L~-1) was added in the culture medium;at the strength seedling phase,the highest tissue culture seedling yield was produced as added 6-BA(0.25～0.27 mg·L~-1) and NAA(0.10～0.15 mg·L~-1) to the culture medium;And at the rhizogenic phases,the rate of rhizogenesis reached to 95% when adding macro-element with concentration of 40%,and that had no any influence to the future growth.After third generation of subculture,the concentration of ABT and NAA could be decreased to 0.5 mg·L~-1 and 0.5 mg·L~-1 respectively for best rhizogenesis.更多还原