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二苯并噻吩降解菌筛选及16S rDNA序列分析
Isolation of Dibenzothiophene-degrating Strain and Its 16S rDNA Sequence Analysis
【摘要】 以DBT(dibenzothiophene,二苯并噻吩)为模型化合物,分离得到了一株能降解DBT的微生物HB2,应用HPLC对其脱硫特性进行了检测。应用PCR技术克隆到16SrDNA片段,核苷酸序列分析结果表明,该菌的16SrDNA全序列与假单胞菌存在98%的同源性,初步确定该菌在微生物系统发育学上的地位。
【Abstract】 Dibenzothiophene(DBT),the typical compound of sulfur-containing organic molecules found in fossil fuels,can be utilized by a strain called HB2,which was isolated in the lab.PCR technology was then used to amplify its 16S ribosomal DNA,i.e.,rDNA,which was found to be homogeneous as much as 98% to Pseudomonas;therefore,basically the position of the strain in microbiological phylogeny was set up.
【基金】 湖北省自然科学基金资助项目(2002AB092);湖北省中药生物技术重点实验室基金
- 【文献出处】 环境科学与技术 ,Environmental Science & Technology , 编辑部邮箱 ,2006年03期
- 【分类号】X172
- 【被引频次】4
- 【下载频次】198