【摘要】 背景与目的肺癌的易感性研究多集中在单个基因的多态性方面,本研究观察了广州地区汉族人群细胞色素P4502E1(CYP2E1)和1A1(CYP1A1)的基因多态性与肺癌的关系,分析其联合作用。方法采用聚合酶链反应和限制性片段长度多态性技术检测91例初发肺癌患者及91例非肺部疾患同性别患者的CYP2E1和CYP1A1的基因多态性,并分析比较。结果在两组间平衡吸烟的因素后,CYP2E1和CYP1A1基因多态性分别单独分析时,与肺癌关系较弱,其OR值分别为5.48和1.51,与对照组之间的差异无显著性(P>0.05)。两者联合分析时,CYP2E1野生型和CYP1A1突变型联合者的OR值为3.00,95%CI为1.03~8.78,其差异有显著性(P<0.05)。结论CYP2E1和CYP1A1单一基因的多态性不能增加患肺癌的危险,而两者联合作用时则可增强患肺癌的风险。更多还原

【Abstract】 Background and objective Most of the researches about lung cancer have focused on the polymorphisms of single gene rather than the cooperation of the genes. The aim of this study is to investigate the correlation of gene polymorphisms of cytochrome P450 2E1 (CYP2E1) Rsa Ⅰ and cytochrome P450 1A1 (CYP1A1) independently and in combination with the risk of lung cancer. Methods A case-control study which included 91 lung cancer cases and 91 hospital-based controls was carried out, and all of them were the Han nationality in Guangzhou district. Blood samples were collected for detecting CYP2E1 Rsa Ⅰ and CYP1A1 gene polymorphisms by polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP). Results No significant difference was observed in genotype frequencies of CYP2E1 Rsa Ⅰ and CYP1A1 Msp Ⅰ between case and control groups. Odd ratio was 5.48 and 1.51 respectively without significant difference (P>0.05). Individuals with CYP2E1 Rsa Ⅰ C1C1 and CYP1A1 m2m2 genotype had 3.00-fold risk of developing lung cancer compared with those who had CYP2E1 Rsa Ⅰ C1C2 and CYP1A1 m1m1 genotype (P<0.05, 95% CI 1.03--8.78). Conclusion Single polymorphism of CYP2E1 and CYP1A1 doesn’t increase the risk of developing lung cancer, but the combination of two genetic polymorphisms significantly increases the risk of developing lung cancer.更多还原