【摘要】 背景与目的研究表明,不均一核糖蛋白(hnRNP)A2/B1在肺癌组织中表达明显增高。本研究旨在探讨hnRNPB1在肺癌患者痰脱落细胞中的表达及其在诊断肺癌中的价值。方法采用免疫细胞化学方法,以特异性hnRNPB1单克隆抗体对70例肺癌患者及30例非肺癌患者痰中hnRNPB1的表达进行了研究。结果70例肺癌患者中痰细胞学检测阳性27例,诊断肺癌的敏感性和特异性分别为38.6%和100.0%。70例肺癌中有50例hnRNPB1表达阳性,29例鳞癌中23例表达阳性,26例腺癌中17例表达阳性,15例小细胞癌中10例表达阳性,诊断肺癌的敏感性为71.4%,特异性为93.3%。hnRNPB1免疫细胞化学染色诊断肺癌较痰脱落细胞检查更敏感,两者具有显著性差异(P<0.05)。结论肺癌患者痰脱落细胞中hnRNPB1表达明显增高,其诊断肺癌的敏感性明显优于痰脱落细胞学检查。更多还原

【Abstract】 Background and objective The expression of heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 increases significantly in lung cancer tissues. The aim of this study is to investigate the expression of hnRNP B1 in exfoliative cells in the sputum of patients with lung cancer and its sensitivity and specificity in diagnosis of lung cancer. Methods The expression of hnRNP B1 in sputum was detected by immunohistochemistry (IHC) using anti-hnRNP B1 monoclonal antibody in 70 patients with lung cancer and 30 normal subjects (control group). Results The results of sputum exfoliative cytology were 27 positive in 70 samples diagnosed with lung cancer, whose sensitivity and specificity were 38.6% and 100.0% respectively. The results of sputum immunohistochemistry were 50 positive in 70 samples, with 23 positive in 29 squamous cell carcinoma and 17 positive in 26 adenocarcinoma and 10 positive in 15 small cell lung carcinoma. Sputum IHC of hnRNP B1 gave a sensitivity of 71.4% and specificity of 93.3%. Sputum IHC of hnRNP B1 was more sensitive than sputum exfoliative cytology (P<0.05). Conclusion hnRNP B1 overexpresses in sputum exfoliative cells and its sensitivity is better than sputum exfoliative cytology in diagnosis of lung cancer.更多还原