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猪链球菌2型广西GX01株Cps2J基因克隆与序列分析
Sequencing and Analysis of Cps2J of Streptococcus suis Serotype 2 GX01 Strain
【摘要】 将分离到的广西猪链球菌2型,用针对猪链球菌2型Cps2J序列的引物Cps1/Cps2进行扩增,得到与预期相符的675 bp的片段,将PCR产物回收连接转化后测序,得到了675 bp的核苷酸序列,经同源性分析表明,GX01株Cps2J序列与猪链球菌2型国内外的毒株核苷酸同源性在98.7%~100%之间,具有高度的同源性;与猪链球菌1型的同源性只有66.7%。
【Abstract】 The Cps2J of GX01 strain which isolated from pigs of Guangxi was amplified by Cps1/Cps2,then the product of PCR was sequenced and analyzed.The result showed that the Cps2J sequence homologies of GX01 and other of Streptococcus suis type 2,which published in GenBank,were 98.7% to 100%,the homology between GX01 and Streptococcus suis type 1(AF155804) was 66.7%.
【基金】 广西科技厅科技攻关与新产品试制项目(0632002-1)
- 【文献出处】 动物医学进展 ,Progress in Veterinary Medicine , 编辑部邮箱 ,2006年11期
- 【分类号】S852.611
- 【被引频次】1
- 【下载频次】72