【摘要】 以禽流感病毒A/Chicken/Hubei/327/2004(H5N1)免疫Balb/c小鼠,将免疫鼠脾细胞与SP2/0骨髓瘤细胞融合,用血凝抑制试验筛选细胞培养上清,采用有限稀释法对阳性孔进行克隆,3次克隆后获得7株能稳定分泌抗H5亚型禽流感病毒血凝素单克隆抗体的杂交瘤细胞株,分别命名为1C4,1D4,1E12,2E11,4C12,4G2和5E12。细胞培养上清HI效价为24~27,腹水HI效价可达210~218。所有单抗与禽流感H7和H9亚型标准血凝抗原,新城疫病毒和鸡传染性支气管炎病毒无交叉反应。在细胞上的中和试验显示具有较高的中和效价,获得的单克隆抗体可在禽流感流行病学的监测中发挥重要作用。更多还原

【Abstract】 Balb/c mice were immunized with the purified AIV antigens.Splenocytes from the immunized mice were fused with SP2/0 myeloma cells.Positive hybridoma clones were screened by hemagglutination inhibition（HI）,and limited dilution method was performed to subclone the positive clones.After three cycles of subcloning,seven McAbs against the hemagglutinin of H5-AIV were obtained,designated as 1C4,1D4,1E12,2E11,4C12,4G2 and 5E12.The HI titers of culture supernatant and ascites were 2⁴-2⁷ and 2¹⁰-2¹⁸.The seven McAbs had good specificity to H5 subtype of avian influenza virus （H5-AIV）.No cross-reactions were found when H7 amd H9 subtypes of avian influenza virus,Newcastle disease virus（NDV） and Avian infectious bronchitis virus（IBV） were tested.All McAbs had high neutralization titers.更多还原