【摘要】 为了比较不同的激活方法对卵母细胞孤雌激活的影响,以及培养液(CR1aa)中添加不同类型的血清对孤雌激活胚体外发育的影响。采用Eth+6-DMAP、A23187+6-DMAP和Ion+6-DMAP三种方法激活山羊卵母细胞,Eth+6-DMAP组孤雌激活胚的卵裂率和囊胚率(35.2%和4.8%)显著低于A23187+6-DMAP组(68.9%和24.1%)和Ion+6-DMAP组(88.1%和48.0%),表明以Ion+6-DMAP激活最为理想。在培养液(CR1aa)与颗粒细胞共同培养条件下,分别添加100 mL/L的发情牛血清(OCS)、胎牛血清(FBS)和新生牛血清(NCS)。添加OCS和FBS后,孤雌胚囊胚率分别为48.1%和45.0%,显著高于添加NCS组(26.0%)。表明OCS、FBS和NCS能有效的提高孤雌胚的体外发育能力,尤其是对提高孤雌胚的囊胚发育能力更佳。更多还原

【Abstract】 The method of parthenogenetic oocytes of goat was studied.Oocytes of goat were activated by using Eth + 6-DMAP、A23187+6-DMAP and Ion+6-DMAP.The cleavage rate and the blastocyst rate of oocytes activated by Eth + 6-DMAP(35.2%,4.8%)were significantly lower than that by A23187+6-DMAP(68.9%,24.1%) and Ion+6-DMAP(88.1%,48.0%).When embryos were co-cultured with GCs in media CR1aa,the same concentration(10%) OCS,FBS and NCS were added.When embryos co-cultured with GCs in media CR1aa,the blastocyst rate of parthenogenetic embryo added OCS and FBS(48.1%,45.0%) were significantly higher than that added NCS(26.0%).It was concluded that it could significantly increase development of goat parthenogenetic embryos by adding the same concentration(10%) OCS,FBS and NCS in media CR1aa.The blastocyst rate of parthenogenetic embryos were significantly increased when added 10% OCS,10%FBS and 10% NCS,nevertheless,10% OCS and 10% FBS were better.更多还原