【摘要】 RNA酶存在于各种环境中,人和动物的唾液、汗液和血液中含有大量的RNA酶,RNA酶很容易使RNA分解,导致在RNA病毒检测中出现假阴性的结果。利用复合核苷氧钒复合物(RVC)去除样品中的RNA酶,结果表明样品中含有超过0.04 ng量的RNA酶,就会影响其检测结果;在RNA纯化时中和多余的RVC后,不影响RNA的体外转录;终浓度为10 mmol/L的RVC可以抑制40μg RNA酶的作用。该方法操作简便,需时较短,成功解决了RNA抽提中RNA酶的干扰,非常适合于实验室使用。更多还原

【Abstract】 RNase were successfully eliminated from samples by Ribonucleoside Vanadyl complexes and reverse transcript was not interfered in vitro while RVC was treated by EDTA.The result indicated RNA could decompose by RNase and did not amplify by RT-PCR while the amount of RNase in sample was more than 0.04 ng,and the anount of 40 μg of RNase in sample could be inhibited by a concentration of 10 mmol/L of RVC.This method was convenient and effective,it successfully removed RNase interference in RT-PCR.更多还原