节点文献

禽流感病毒HA1基因的克隆及原核表达

Cloning and Prokaryotic Expression of HA1 Gene of AIV

推荐 CAJ下载
PDF下载
不支持迅雷等下载工具，请取消加速工具后下载。

【Author】 ZHOU Xue-mei~(1), HUO Hui-ling~(2), ZHANG Biao~(3),LI Yong-qing~(4),WANG Lian-qun~5(1.Department of Veterinary Medicine, China Agricultural University,Beijing, 100094,China;2.Hebei province Institute of Veterinary Drug Control .Shijiazhuang Hebei 050051,China;3.Department of Veterinary Medicine, Hebei Agricultural University, Baoding,Hebei,071000,China;4.Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100089,China;5. Handan Animal Quarantine Station,Handan,Hebei, 056001,China)

【机构】中国农业大学动物医学院；河北省兽药监察所；河北农业大学动物医学院；北京市农林科学院畜牧兽医研究所；邯郸市动物检疫站北京100094；河北石家庄050051；河北保定071000；北京100089； 5.邯郸市动物检疫站；河北邯郸056001；

【摘要】用RT-PCR方法扩增出禽流感病毒A/Muscovyduck/Fujian/2/2000(MF00)株部分HA基因,大小约900bp,将其克隆到pMD18-T载体上,并进行了序列的分析。通过BamHⅠ和XhoⅠ酶切位点将HA基因亚克隆到原核表达载体pGEX4T-3上,成功构建了阳性重组表达质粒,并将阳性重组质粒转化BL21(DE3)菌株。经IPTG诱导表达,结果禽流感HA基因在pGEX4T-3系统中得到高效表达,经Westernblotting检测证实表达产物有生物学活性。更多还原

【Abstract】 In this study, a 0.9 kb DNA fragment of HA1 gene of AIV A/Muscovyduck/Fujian/2/2000(MF00)strain was obtained by RT-PCR. The HA gene was inserted into the pMD18-T vector and then sequenced .Then the HA1 gene was cloned into the expression vector of pGEX4T-3 by restriction enzyme (BamHⅠand XhoⅠ).The recombinant plasmid was transformed into E.coli(BL21/DE3)and induced with IPTG.The results showed that the HA1 segment was highly expressed in E coil(BL21/DE3) .Western blotting analysis showed that the recombinant protein shared good antigenity.更多还原

【关键词】禽流感病毒； HA1基因；克隆；原核表达；
【Key words】 Avian influenza virus； HA1 gene； cloning； prokaryotic expression；

【基金】河北省自然科学基金(C2005000654)

【文献出处】动物医学进展 ,Progress in Veterinary Medicine , 编辑部邮箱 ,2006年01期

【分类号】S852.65
【被引频次】8
【下载频次】280

知网节下载

节点文献中：

本文链接的文献网络图示:

本文的引文网络

节点文献