【摘要】 目的建立一种比现有方法敏感、准确性高、重复性好的人类mdr1基因的新型实时荧光定量PCR检测新方法。方法用Primer Express 2．0引物设计软件设计引物和MGB探针,以Taq Man-MGB探针技术为基础,运用Taq Man-MGB探针,以含有目的基因mdr1cDNA的质粒pHaMDR1／A为阳性模板,建立实时荧光定量PCR检测方法。结果所建立方法的最低检测限度为15个基因拷贝／反应,在待扩增DNA浓度为3．061×103 cps／ml-3．061×109cps／ml范围时,模板浓度与循环阈值(Ct)之间的相关性良好,决定系数r2为0．988243。结论应用Taq Man-MGB探针的实时荧光定量PCR方法检测人类mdr1基因,具有灵敏度高、特异性高和精确性高等优点。更多还原

【Abstract】 Primer Express 2.0 software was used to design the primers and the MGB probe. With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061×103 to 3.061×109 cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value. This PCR method allows sensitive, specific and quantitative detection of human mdr1 gene.更多还原