【摘要】 目的研究比较链霉亲合素(SA)-DTPA-Gd经腹腔和静脉注射后对荷瘤裸鼠体内肿瘤的强化效应,以提高磁共振(MR)靶向成像的敏感性。方法 12只荷人结肠癌裸鼠经尾静脉注射生物素化单抗600μg,24 h后腹腔注射80μg亲和素,30 min后分别经静脉和腹腔给予SA-DTPA-Gd。分别在给药前和给药后20、60 min和3、6、9、12、24 h进行MR 扫描并记录比较肿瘤和肝脏的信号强度。结果静脉组和腹腔组中肿瘤的最大强化率分别为70．2％和46．4％,两者具有显著性差异;静脉组和腹腔组中肝脏的最大强化率分别为20．4％和23．7％,两者间差异不显著。结论生物素化单克隆抗体和SA-DTPA-Gd对肿瘤具有特异性靶向增强作用;血药浓度较高是静脉注射SA-DTPA-Gd比腹腔注射更有利于皮下肿瘤强化的原因。更多还原

【Abstract】 Objective To investigate the effect of streptavidin (SA)-DTPA-Gd after intraperitoneal and intravenous administration for tumor enhancement in targeted magnetic resonance imaging (MRI). Methods Biotinylated monoclonal antibody CL3 (600 μg) was intravenously injected into 12 BALB/c nude mice with subcutaneous inoculation of LoVo cells, followed by administration of 80 μg avidin as the chaser 24 h later and then SA-DTPA-Gd was injected intravenously or intraperitoneally after another 30 min. MRI was performed before and 20, 60 min and 3, 6, 9, 12 h after the injection of the contrast agents, and the MR signal intensity of the tumor and liver was determined. Result The maximum enhancement ratio of the tumor was 70.2% in the intravenous injection group and 46.4% in the intraperitoneal group, showing significant difference between them. The maximal enhancement rate of the liver was 23.7% in the intraperitoneal group and 20.4% in the intravenous group, showing no significant difference. Conclusion MR targeted imaging with biotinylated monoclonal antibody CL3 and SA-DTPA-Gd has specific enhancement effect. Higher blood level of SA-DTPA-Gd in the intravenous group facilitates the tumor enhancement in MRI in subcutaneous tumor model.更多还原