【摘要】 目的黄绿青霉素的合成。方法黄暗青霉菌接种于已高压灭菌的大米中混匀,培养数日后,将霉变大米的乙醇提取液,旋转蒸干,溶于苯,浓缩后得到的粗毒素在硅胶柱上层析,依次用苯、乙醚、乙酸乙酯、丙酮洗脱,收集乙酸乙酯部分浓缩,4℃放置2 d后结晶。结果所得产物经高效液相色谱检测成分单一,纯度高。结论用大米做培养基产量高,杂质少;新的柱层析法易分离,利结晶。更多还原

【Abstract】 Objective?The production of citreoviridin.Methods Innoculate sterilized rice with Penicilliun Citreo-Viride and mix them together.Cultivate for several days then extract the moldy rice with ethanol.The extract is dissolved with benzene then concentrate it at 60℃.The crude toxin is added to a Silica Gel colume.The colume is eluted sequentially with benzene-ethyl-ethyl acetate-acetone.The ethyl acetate fraction is reduced in volume,and the toxin crystallized after 2 days at 4℃.Results The production is proved single and pure by HPLC.Conclusions Rice as culture can get higher yield and fewer impurity;the new method of column chromatography is easier to separate and benefit crystallization.更多还原