【摘要】 目的:研究菲立磁标记的大鼠骨髓基质干细胞(BMSCs)在同种异体移植鼠肝脏中的追踪方法.方法:采用菲立磁(Feridex)对分离培养的BMSCs进行标记,Prussianblue染色和电镜观察对标记后的BMSCs进行鉴定;取鉴定后的BMSCs2mL(1×109/L)分别注射到SD异体大鼠的门静脉、肝局部实质、尾静脉内;采用磁共振成像(MRI)技术(SE-T2WI,FSE-T2WI,GRE-T2WI序列)分别对移植前6h,移植后6h,1,3和5wk时的大鼠肝脏进行扫描.结果:Prussianblue染色证实Feridex标记BMSCs阳性率>90%,透射电镜见铁颗粒位于BMSCs胞质中;MRI扫描SE-T2WI序列成像显示:各移植组在移植前肝脏均未见异常低信号区;门静脉组大鼠移植后6h在肝门部可见异常的低信号改变,1,3wk低信号改变范围逐渐扩大,第5周时低信号改变消失;肝内注射组大鼠移植后6h在肝脏局部注入区域可见异常的低信号改变,1,3,5wk低信号改变区域逐渐扩大.尾静脉组在移植后各时间点均未见到异常低信号改变.结论:Feridex标记的大鼠BM-SCs在同种异体移植鼠肝脏中可用MRI进行有效的追踪.更多还原

【Abstract】 AIM: To study the method of tracking of allogeni- cally grafted rat bone marrow stem cells （BMSCs） labeled with Feridex in rat liver. METHODS:Feridex was used to label iso- lated BMSCs. Labeled BMSCs were examined by Prussian blue staining and electron microscopy. Successfully labeled BMSCs （2.0 mL,1×10⁹/L） were respectively transplanted through portal vein, local liver parenchyma and caudal vein of the SD rats. Six hours before transplantation and 6 h, 1, 3, and 5 weeks after transplantation, magnetic resonance imaging （MRI） examination （the sequences used were SE-T₂WI, FSE-T₂WI, GRE-T₂WI） was conducted on rat livers. RESULTS: Prussian blue staining confirmed the labeling efficiency was above 90%, and transmission electron microscopy showed numerous iron particles in the cytoplasm of Feridex-labeled BMSCs. MRI （SE-T₂WI） showed: no remarkable low signal change was found in the liver images before transplantation of all 3 groups. In the porta hepatis of portal vein transplantation group remarkable low signal changes were seen 6 h after transplantation and the extent gradually expanded 1 and 3 weeks after transplantation, while the low signal change could not be seen 5 weeks after transplantation. In the section where the cells were injected in of liver topical transplantation group remarkable low signal changes were seen 6 h after transplantation and the extent gradually expanded 1, 3 and 5 weeks after transplantation. No remarkable low signal change was found in the liver images of caudal vein transplantation group at all time points after transplantation. CONCLUSION: Allogenically grafted Feridex-labeled BMSCs can be tracked effectively in rat liver by MRI.更多还原